Surface Display of Recombinant Proteins on Bacillus thuringiensis Spores
نویسندگان
چکیده
منابع مشابه
Surface display of recombinant proteins on Bacillus thuringiensis spores.
The insecticidal protoxin from Bacillus thuringiensis has been shown to be a major component of the spore coat. We have developed a novel surface display system using B. thuringiensis spores in which the N-terminal portion of the protoxin is replaced with a heterologous protein. The expression vector with a sporulation-specific promoter was successfully used to display green fluorescent protein...
متن کاملSurface display of recombinant proteins on Bacillus subtilis spores.
We developed a novel surface display system based on the use of bacterial spores. A protein of the Bacillus subtilis spore coat, CotB, was found to be located on the spore surface and used as fusion partner to express the 459-amino-acid C-terminal fragment of the tetanus toxin (TTFC). Western, dot blot and fluorescent-activated cell sorting analyses were used to monitor TTFC surface expression ...
متن کاملDisplay of recombinant proteins on Bacillus subtilis spores, using a coat-associated enzyme as the carrier.
The display of proteins such as feed enzymes at the surface of bacterial spore systems has a great potential use for animal feed. Feed enzymes increase the digestibility of nutrients, leading to greater efficiency in the manufacturing of animal products and minimizing the environmental impact of increased animal production. To deliver their full potential in the gut, feed enzymes must survive t...
متن کاملSurface display of recombinant proteins on Escherichia coli by BclA exosporium of Bacillus anthracis
BACKGROUND The anchoring motif is one of the most important aspects of cell surface display as well as efficient and stable display of target proteins. Thus, there is currently a need for the identification and isolation of novel anchoring motifs. RESULTS A system for the display of recombinant proteins on the surface of Escherichia coli was developed using the Bacillus anthracis exosporal pr...
متن کاملSurface display of heterologous proteins in Bacillus thuringiensis using a peptidoglycan hydrolase anchor
BACKGROUND Previous studies have revealed that the lysin motif (LysM) domains of bacterial cell wall-degrading enzymes are able to bind to peptidoglycan moieties of the cell wall. This suggests an approach for a cell surface display system in Gram-positive bacteria using a LysM-containing protein as the anchoring motif. In this study, we developed a new surface display system in B. thuringiensi...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2005
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.71.6.3337-3341.2005